Guide to information and use of oxalic acid strips

Introduction

Oxalic acid is one of the strong organic acids with the chemical formula C2H2O4. It is a white solid, relatively strong carbonic acid. We find it in nature in concentrations in sorrel (commonly known as nettle) and in many other plants such as black pepper, parsley, poppy seeds, amaranth, spinach, Swiss chard, beets, cocoa.

The mode of action of oxalic acid against Varroa mites has not been fully investigated, but it requires direct contact of the mites with the oxalic acid. It is thought that the oxalic acid acts through direct contact or through the deposition of oxalate hemolymph by destroying the mouthparts of the varroa, which results in it being unable to feed and dying from starvation. The acaricidal effect can be mainly due to the low pH of the respective preparation.

Oxalic acid strips are essentially cut cellulose strips soaked in a complete homogenized solution of Glycerin and Oxalic Acid in the proper proportions. The active substance oxalic acid due to the low Ph has an acaricidal effect against Varroa, however the addition of glycerin significantly prolongs the action of oxalic acid because as a viscous non-volatile substance it binds the acid providing a slow release from the cellulose strips which can accordingly season to range from 25-40 days. The ¨collaboration¨ of glycerin with oxalic acid combined with the appropriate absorbent material (cellulose) provides a high and prolonged effectiveness in a 100% ecological way, which makes it an excellent treatment significantly inhibiting varroa even with the presence of brood in the colony with efficiency rates <90%.

  • It is 100% Ecological – Organic Treatment.
  • It is quite effective (with proper application <90%)
  • It is low cost, sustainable something important for professional beekeepers
  • It is easy to use, without repeated applications being necessary.
  • Does not pose a risk to human health
  • Can be used as a treatment repeatedly more than once in a year in different months.
  • Has not be observe Varroa becoming immune to Oxalic Acid.

 

Disadvantages:

  • Like any organic acid, in periods of insufficient melitophoria, bad weather conditions or overdose it can affect the Queen’s birth rate.
  • In some cases the bees may create “dimples” in the frames due to their disturbance from the strips. (This is temporary for a few days)
Oxalic Acid Bees

Use / Application

How should I use the oxalate strips?

Oxalic acid strips are suitable for a wide range of temperatures from 15 -35 degrees Celsius. Since oxalic acid works by contact, below 15 degrees we have low movement of bees in the frames and therefore less contact of different bees with the tapes, which delays and perhaps reduces the effectiveness of the treatment with possible negative consequences in failure. At temperatures above 35 degrees we have a faster release of oxalic acid from the strips with possible negative consequences for the health of the colony. In summary, we avoid using Winter in low temperatures and during periods of intense heat.

The number of strips placed per colony depends on its population size. Below is a helpful table:

BEE POLULATION FRAMES (langstroth) STRIPS
    3 ½  (Hanging)
   4-5 1
   6-7 2
   8-9 3
    10 4
  <11 5
   

Especially during the spring period when the beehives is in development, we are careful not to apply more strips than necessary, we do not want to “pressure” the bee by affecting its development.

Oxalate strips to be effective must ALWAYS be placed in the frames where brood is present. This is due to the fact that Varroa reproduces in the brood and young bees and queen bees always have the highest infestation and since the oxalic acid works in contact in this way we maximize the spread of the active substance in the infected population. However, we do not need to place absolutely in the center of the frame disturbing the movement of the bees. We can place them in a ¨Λ¨ formation 5-7 cm from the imaginary center of the frame and alternately. We fold the strips and “wear” them in the frame that has a brood.

Also we can cut them in half and place 2 halves hanging with a toothpick, instead of one whole folded. The difference is that when they are half balanced in the gap of the frames it leaves a better corridor for the bees and the queen to pass through. It also reduces “dimpling” of the wax in the frames. However, the disadvantage is that it takes significantly more time to place them in the colonies.

Although nothing changes in efficiency, the beekeeper will personally decide which position serves him best.

INDICATIVE PICTURES OF INSTALLATION OF OXALIC TAPES

Although in beekeeping there are exceptions and from season to season the parameters change, as a general rule we set the moments when the temperatures are suitable and especially when the colonies have the least brood for each season. This is usually right after a honey harvest. Our apiaries to fill with honey, reduce the food to the queen to reduce the brood rate, as a result they keep fewer frames with brood and many more with honey. Practically then, the varroa has fewer brood cells to enter and “cover”, so it is more vulnerable and necessarily crowded into a smaller space (4-0 maybe, frames) to reproduce within the hive. At those moments the strips of oxalic acid make a “great work” with a rate that reaches <90%.

An important think that the beekeeper must have in mind is that the treatment must be applied on the day of harvest or in the next 2-3 days. Why;

Because after a honey harvest the colony tends to increase the queen laying rate, especially if we practice nomadic beekeeping and move our hives to area with blossom for a re-growth of the Beehive. Given that:

1) Usually 2-3 days after harvest beehive organized and the queen start lays eggs again

2) That the day just before the brood is sealed the varroa enters the cell

it means we have about 10 days where the varroa is “severely exposed”. In these 10 days that should ALREADY have the treatment applied at the earliest to kill the mite varroa at higher rates. If we do not do timely treatment and we are late, then after 10 days the varroa has already entered in brood and the application of the treatment  is less effective and also the varroa has already done damage to the colonies without us being able to do anything. A good beekeeper does not miss the right timing, this one is VERY SERIOUS.  We take advantage of these opportune moments!

Normally only one course of treatment is needed. However, there are some cases where it may be necessary to repeat the treatment with new strips after 15-20 days.

This is needed in the following 2 cases:

  1. A) If the varroa infestation is particularly high in our colonies. Eg a colony with 1,000 varroa infested, even if 90% of the mites are killed, the remaining 10% is 100 varroa. These 100 varroa if we don’t reduce them with 2 applications then in a few months they will reach close to their original number again and we don’t want that. Of course, in order to realize this, we have to measure the varroa fall over a period of 20 days in beehives ¨indicators¨ and if the sum is high enough, a second application is needed. Also with the alcohol test method we can have an indication of the rate of varroa infestation before the treatment.

*(For more information on varroa growth rate see section: Varroa Reproduction and Life Cycle (Examples).

  1. B) If some strips are destroyed in 15 days by the bees. This happens ussaly in large bees because of the cleaning instinct, then we don’t necessarily do 2 application cycles, we can just selectively replace the damaged tapes with new ones.

In most cases where an application cycle is performed the oxalate strips should remain in the cell for up to 30 days. Then if they haven’t already been destroyed by the bees, we have to remove them.

However, when there are 2 treatment cycles then we can make them shorter in 20 days for the first application and another 20 days for the second application. A total of 40 days.

In the offshoots it is a very good opportunity to clean them almost completely of the varroa by doing the following tactic:

Counting 15 days from the day we created the offshoot we apply ½ -1 strip of oxalate according to size of new colony (2-5 frames) for only 20 days. During this time the scion is almost broodless because most of the brood has already come out from the frames and the new queen will not lay eggs yet. So the varroa is almost completely exposed to the action of the oxalic acid strips. With this tips we achieve very good fighting results with minimal financial costs and give our new bee a very good and “clean”start!

Biological Method / Safety

No risk of contaminating honey or wax, in fact oxalic acid is a component of honey and belongs to the organic compounds. That is why the method of managing varroa with oxalate strips has a biological approach.

As with any kind of treatment, oxalic strips show some bee toxicity. It is estimated that oxalic acid is 50-70 times less toxic to bees than to the Varroa mite. Due to its toxicity to bees as an acid it can shorten the life of the bee by delaying the progress of the hive, so it should be used appropriately as much as possible in the right season and weather conditions. Abuse and overdoses may not have negative effects on the health and development of our colonies.

Oxalic acid in this form and at ambient temperatures does not present any danger to human health, in contrast to chemical preparations as well as to the method of oxalic sublimation which without special protective measures can cause serious damage to the lungs and eyes . However, we recommend doing the treatment after wearing disposable gloves and of course not bringing the oxalate strips into contact with our eyes and mouth.

Advantages:

  • It is 100% Ecological – Organic Treatment.
  • It is quite effective (with proper application <90%)
  • It is low cost, sustainable something important for professional beekeepers
  • It is easy to use, without repeated applications being necessary.
  • Does not pose a risk to human health
  • Can be used as a treatment repeatedly more than once in a year in different months.
  • Has not be observe Varroa becoming immune to Oxalic Acid.

 

Disadvantages:

  • Like any organic acid, in periods of insufficient melitophoria, bad weather conditions or overdose it can affect the Queen’s birth rate.
  • In some cases the bees may create “dimples” in the frames due to their disturbance from the strips. (This is temporary for a few days)
Varroa

Varroa Physiology/ Beekeeper's Goal

The varroa parasitizes adult bees and feeds on the bee’s hemolymph. The reproduction and life cycle is as follows:

1) The varroa enters a cell 15-20 hours before being sealed.

2) The mite is found in the larva’s food.

3) The mite feeds on the protonymph.

4) The female varroa lays the first egg (male), 60 hours after the cells are sealed and continues to lay eggs for a period of 30 hours.

5) 1-6 eggs develop into larvae, proto-nymphs and second-nymphs. In 5-6 days the males are adults, in 7-8 days the females are adults.

6) The male mates with the females inside the cell.

7) The fertilized females leave the cell together with the bee. The male dies.

8) Mites go from bee to bee for 2-15 days and then enter a hive to lay eggs.

We should also know the mathematical growth rate of varroa per generation:

1.6 when giving birth in a female bee cell

2.6 when it gives birth in a male bee (drone) cell

The difference is due to the fact that the drone hatches 28 days while the worker bee 21 days. So the new varroa has +7 more days to mature its progeny with better chances of survival and clearly more hemolymph due to larval size of drone(Male Bees). For these 2 reasons the varroa prefers to reproduce in Male Bees cells.

Unfortunately, due to its high reproduction rate and the natural protection provided by the bee brood coating, the control and suppression at low levels is a challenge.

There are the following ways:

  1. a) To use “Varroa Control Device”

The reliable way is to place about 300 bees in this special cup and add alcohol by shaking the cup well for about 1 minute. The alcohol also separates the varroa from the bees and at the bottom of the device we count the fallen varroa. If in relation to bees the varroa percentage is greater than 4-5% (Varroa/Bees) it means that treatment is needed

  1. b) A simple method is to carefully cut with a good blade the mature brood “cover” membrane (15-19 days old) into a square area of 8 x 8 cm. Then tap the frame firmly on a clean surface to dislodge the bee larvae from the frame . About 200 young white larvae will come out and on them we can easily count the varroa that are present. In this way we can have an image of the varroa infestation in the brood and a ratio of varroa/200 bee larvae.
  2. c) Another simple way is to apply strips of oxalic acid or Amitraz to 3-4 random hives and watch the varroa fall for 5-10 days. If the total is large eg <200 varoa, although we don’t have an exact projection rate, it means that the mites are enough and our colonies need treatment.

According to experiments that have been done at the Greek Aristotle University of Thessaloniki as well as research universities in Argentina, the oxalic acid strip methods over 6 years and more show stable results without any development of resistance from the Varroa mite. This may be due to the different mode of operation from the chemical preparations that act as neurotoxins (Amitraz, Coumaphos, Flumethrin, Fluvalinate) to which the varroa mutates DNA and develop resistant genomes. This makes the use of organic acids the future in the fight against varroa.

Usually the results are sufficient since oxalic acid strips can be 90-95% effective, however this does not mean that it is always enough.

We need to consider the following factors:

  • If the bees keep many broode frames during the treatment season, we may not have this high efficiency, but rather low below 90%. This is the case with any form of therapy. That’s why we always choose the moments when the beehives have as little as possible brood.
  • If even though we apply treatment we are close to infected colonies. The varroa that comes from outside gradually infects our colonies and in 1-2 months we may see varroa in the bees again. Usually harvests in forests where a large number of bees are concentrated in a limited geographical area the phenomenon of external varroa contamination can be great.

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  • If we have a very high initial rate of varroa infestation. If in a colony we have, for example, 1500-1000 varroa and we reach 90% effectiveness by applying treatment, another 100-150 varroa will remain alive. These are enough after a while to re-establish a large percentage of infestation because the reproduction rate of Varroa is quite fast.

For all the above cases there may one  cycle treatment not enough and a second cycle of treatment mayde needed. This happens by exception and the beekeeper himself will decide if it is necessary.

The best strategy is to keep varroa at low levels and not trying to  eliminate to get to zero.

Trying to eliminate varroa is not realistic because in the presence of a beekeeping season it is very difficult and multiple applications of treatments will be needed which will stress our bee colonies quite a lot and increase the monetary cost. Also, there is no way to stop the influx of varroa from the outside, which means that no matter how clean our bees are, in a short time they will again be infested with varroa from outside.

So the best course of action is to keep varroa under control with effective treatment times, keeping the mite population low enough to not affect the health of our colonies.

General Conclusions: Costing / Comparison / Evaluation

Approximately for beekeeping in Greece between March and October,  1 application is needed in the Spring, 2 for the summer and 1 application in the Autumn. Approximately (2+5+3+2)= 12 x 2 cycle  = 24 strips approximately per colony.  Smaller non-productive colonies due to size and due to less varroa contamination from external factors (other neighboring colonies) due to non-movement have 30-50% lower treatment costs.

Although each form of treatment has its advantages and disadvantages, oxalic acid strips  present significant advantages compared to many existing treatments.

  • Low cost for professional beekeepers
  • High efficiency
  • Extended duration of activity even with broode
  • Biological control of Varroa
  • Harmless to human health compared to oxalate sublimation or chemical formulations
  • Quick and easy application in our colonies
(VERY IMPORTANT GET UPDATED)

Oxalate strip guide program, customized for Greek beekeepers. However, they can also be adapted on a global scale depending on the climatic conditions of each country

The program below is indicative and adapted according to the climate and beekeeping activities of  Greece. Obviously  modifications can be made according to the current needs, however the following advantages are presented if you follow it.

Its aim is:

  1. A) To effectively control the varroa mite through prevention and control.
  2. B) To reduce as much as possible the cost of treatment applications
  3. C) To adapt appropriately to the beekeeping parametre of Greece
  4. D) To be simple and quick at application time

We choose this period because the temperatures reach 17-20 degrees Celsius so it is a suitable temperature to apply oxalic acid strips to beehive between 6-8 frames population.

We apply a maximum of 1-2 strips of oxalic acid from 20-30 days to reduce the number of mites from those that survived the Winter as well as external contamination from other Bees.

In beehives under 6 frames we do not put the spring season in order not to negatively affect the development of the bee season. We also do not let the beehive reach 10 frame population without applying oxalate strips because at this size it will maintain 7-8 frames, so the effectiveness of the treatment will be reduced if the varroa go inside in so much brood cells.

We should keep in mind about the appropriate circumstances. If, despite the season and the month, there is prolonged cold and rain, it is better to wait a bit to stabilize the weather.  We don’t want to burden our colonies with the wrong application of treatment in the growing season.

Usually within this time period we have the Forest honey harvests, so we have less frames (5-2 frames) and more honey.

So having favorable conditions and as long as the upper temperatures do not exceed 35 degrees Celsius, we apply oxalate strips to the beehives where we have already collected the honey.

Usually the colonies are on 2 floors at that time, so during the harvest we organize the hive properly by taking down to the first floor all the brood frame and placing 4 strips of oxalate in the hive. We also place another strip on the upper floor (if it has one) in a central frame. In total, depending on the size of the colony, we apply 4-5 strips of oxalate for 30 days.

*IMPORTANT: It will be better for convenience and time saving to apply the oxalate strips during our harvest so that we do less operations on our bees. And also if not on the day of harvest it should be within the first 3 days after that to give the strips enough time to kill the varroa while it is exposed. If we delay and the queen increase the spawning rate (More new Brood) there is a risk through our own negligence  the varroa entering the new brood making them infected and even if treatment is applied afterwards,  the damage has been done and the effectiveness will not be as high. (See section : D) D) When are the “perfect” moments to Varroa treatment? (IMPORTANT)”

Usually in this time period we have the Pine and sunflower harvests, so we have very little frame broode because is full honey, a good opportunity to fight the varroa.

Usually the colonies are smaller than in May and June at that time, so during the harvest we place 3-4 strips of oxalate for 30 days in the central frames of the bee. Also, because in the months of August-September has been observed bigger entry varroa from other neighboring colonies , we monitor and count in some of our hives the sum of the killed varroa and if it is big enough (eg 300, 500, 800 varroa)  maybe repeat one more treatment cycle in first 20 days with perhaps fewer strips (20+20=40 Days).  It is perhaps the most critical application in the month of August because the varroa must be reduced enough so that the following autumn will be as “clean and sanitary” from varroa as possible. So need Attention!

*IMPORTANT: It will be better for convenience and time saving to apply the oxalate strips during our harvest so that we do less operations on our bees. And also if not on the day of harvest it should be within the first 3 days after that to give the strips enough time to kill the varroa while it is exposed. If we delay and the queen increase the spawning rate (More new Brood) there is a risk through our own negligence  the varroa entering the new brood making them infected and even if treatment is applied afterwards,  the damage has been done and the effectiveness will not be as high. (See section : D) D) When are the “perfect” moments to Varroa treatment? (IMPORTANT)”

Usually around this time we have one more time again Pine honey harvests, so we have the last treatment application we will do with oxalic acid strips as the temperatures are close to 17-20 degrees celsius and any further drop in temperature will not allow the use of oxalate strips.

Usually the colonies are still smaller than in the spring and summer at that time, so during the harvest of the Fir we place 1-2 strips of oxalate for 30 days in the remaining brood frames. After the bees they have low varroa level to “welcome” in a few weeks the Winter as they gradually reduce their brood .

From mid December – January the temperatures are quite low 3-10 degrees Celsius and our bees no longer keep brood. This will be the last and important treatment to close the beekeeping season.

Any remaining varroa is now completely exposed. We should not apply oxalate strips again due to low temperatures, so we recommend applying 2 sublimations of oxalic acid or 1 cycle of treatment with approved amitraz strips (Apivar –Apitraz) if we do not practice organic beekeeping. This way we will reach almost zero varroa percentage in our colonies.

*IMPORTANT: Keep in mind that even though it is Winter, when the right treatment is applied and the conditions and temperature are not as high as possible in the following days (e.g. <10 degrees) so that the bees can move for some hours in the hive and especially at lunchtime hours when the temperature rises not to be a “tight ball” due to low temperatures, so that the treatment has maximum effectiveness and does not negatively affect the health of the bees.